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Core parameter correspondence table for chromatographic silica gel selection and separation purification

The filler has an average particle size of 90μm and 34μm, with the column tube composed of PP/PMMA/glass.

classify Specific conditions/measures Select/meaning
Molecular weight and filler pore size Molecular weight <500Da The packing hole diameter is 6nm-12nm
Molecular weight 500-2000Da The packing hole diameter is 12nm-30nm
Molecular weight 2000-10kDa The filler pore size is 30nm-50nm
Molecular weight 10k-40kDa The filler pore size is 50nm to 100 nm.
Molecular weight 40k-100kDa The filler pore size is 100nm-200nm
Pressure-Filler-Column Tube Medium and low pressure (<1.3MPa) -Silicone Filling material: 20-100 μm (spherical or irregular), PP/PMMA/glass column tube
Medium and low pressure (<0.3MPa) -Agarose/Glucan The filler has an average particle size of 90μm and 34μm, with the column tube composed of PP/PMMA/glass.
Medium and low pressure (<1.3MPa) -Polymer Filling material: 30-40 μm spherical particles; column tube: PP/PMMA/glass
High pressure (<30MPa) -Silicone/Polymer Filling material: 5-20 μm spherical, stainless steel column tube
Mesoporous silica Applicable pressure <10MPa
Sample characteristics and chromatographic types Molecular weight> 2000, insoluble in water Non-aqueous mobile phase exclusion chromatography
Molecular weight <2000, soluble in water Mobile phase exclusion chromatography
Insoluble in water, isomers/isomers Partition chromatography/adsorption chromatography
Soluble in water and dissociable (alkali) Cation Exchange Chromatography
Soluble in water, dissociable (non-alkali) anion-exchange chromatography
Soluble in water, ionic and non-ionic mixture Reverse ion-pair chromatography
Silica gel column fixed key indicators distribution of pore size Select the molecular weight of the corresponding product
Specific surface area and particle size distribution Number of corresponding separation plates
Specific surface area, moisture Corresponding to the strength of polarity; modified indicators correspond to the strength of non-polarity
Container volume, pH value, silica purity, metal ion impurities Affects stationary phase properties and separation efficiency

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